Author(s): Qian, X., Hou, J., Wang, Z., Ye, Y., Lang, M., Gao, T., Liu, J. and Hou, Y.
Journal: Scientific Reports
There is high demand for forensic pedigree searches with Y-chromosome short tandem repeat (Y-STR) profiling in large-scale crime investigations. However, when two Y-STR haplotypes have a few mismatched loci, it is difficult to determine if they are from the same male lineage because of the high mutation rate of Y-STRs. Here we design a new strategy to handle cases in which none of pedigree samples shares identical Y-STR haplotype. We combine next generation sequencing (NGS), capillary electrophoresis and pyrosequencing under the term ‘NGS+’ for typing Y-STRs and Y-chromosomal single nucleotide polymorphisms (Y-SNPs). The high-resolution Y-SNP haplogroup and Y-STR haplotype can be obtained with NGS+. We further developed a new data-driven decision rule, FSindex, for estimating the likelihood for each retrieved pedigree. Our approach enables positive identification of pedigree from mismatched Y-STR haplotypes. It is envisaged that NGS+ will revolutionize forensic pedigree searches, especially when the person of interest was not recorded in forensic DNA database.
Source Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595879/
Peoples: - | Places: - | Topics: capillary electrophoresis, FSindex, large-scale crime investigations, next generation sequencing (NGS), and pyrosequencing | DNA Type: Y-DNA